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GenScript corporation
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aes containing a kanr cassette and the gfp gene optimised for its expression in mycobacteria (egfp) Aes Containing A Kanr Cassette And The Gfp Gene Optimised For Its Expression In Mycobacteria (Egfp), supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/aes containing a kanr cassette and the gfp gene optimised for its expression in mycobacteria (egfp)/product/GenScript corporation Average 90 stars, based on 1 article reviews
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GenTarget
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Shanghai GenePharma
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Shanghai GenePharma
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Image Search Results
Journal: Scientific Reports
Article Title: Non-canonical integration events in Pichia pastoris encountered during standard transformation analysed with genome sequencing
doi: 10.1038/srep38952
Figure Lengend Snippet: ( A ) Disruption of an untargeted gene by a GFP expression cassette fused to E. coli KRX DNA ( B ) Disruption of an untargeted gene by a re-integrated AOX1 locus. ( C ) Co-integration of E. coli KRX DNA in fusion with an expression cassette at the AOX1 locus.
Article Snippet: In brief, a linearized
Techniques: Disruption, Expressing
Journal: Scientific Reports
Article Title: Non-canonical integration events in Pichia pastoris encountered during standard transformation analysed with genome sequencing
doi: 10.1038/srep38952
Figure Lengend Snippet: ( A ) After transformation the linear GFP expression cassette from pAHBgl-GFP moved to chromosome 4, where the AOX1 locus is situated. ( B ) Two distal homologous sequences of the cassette with the chromosomal AOX1 locus mediate a double cross-over recombination event ( C ) The cassette has replaced the AOX1 locus on chromosome 4 ( D ) The excised linear AOX1 locus moved to chromosome 2 ( E ) Potentially due to a DSB initiating the NHEJ pathway, the AOX1 locus is integrated into a random gene on chromosome 2 ( F ) The untargeted gene PP7435_Chr2-1130 was disrupted. While the AOX1 locus is still functional, the disruption of the untargeted gene likely led to the observed abnormal colony morphology in the case of JPS014.
Article Snippet: In brief, a linearized
Techniques: Transformation Assay, Expressing, Functional Assay, Disruption